rat anti ca ii  (R&D Systems)

Journal: Cell Transplantation

Article Title: Reversibly Immortalized Mouse Articular Chondrocytes Acquire Long-Term Proliferative Capability While Retaining Chondrogenic Phenotype

doi: 10.3727/096368914x681054

Figure Lengend Snippet: Figure 3. Chondroid matrix production by iMACs in micromass assay. (A) Alcian blue staining of the micromasses formed by iMACs and mesenchymal progenitors. High cell density micromasses derived from iMAC-5, iMEF, or C3H/10T1/2 cells were seeded in multiwell culture plates. Alcian blue staining was performed at 2, 3, and 4 days following plating (a). The stain was then dissolved and quantified for OD595. iMACs demonstrate significantly higher chondroid matrix production (n = 3, p < 0.025). (B) Col2aI immunohistochemical staining was performed on micromass culture. Micromasses formed by the indicated cells were fixed and subjected to anti-collagen type II antibody immunohistochemical staining. Isotype IgG was used as a negative control staining (now shown). Each assay condition was done in triplicate. Representative results are shown.

Article Snippet: Micromasses were stained with rat anti-collagen type II antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), followed by incubation with anti-rat IgG-HRP secondary antibody (Santa Cruz Biotechnology).

Techniques: Staining, Derivative Assay, Immunohistochemical staining, Negative Control